Donna Murphy

Murphy dropped out of the New York University drama program in her sophomore year when she was cast to understudy the three backup singers in the 1979 Broadway musical They're Playing Our Song. In a 2007 interview, Murphy explained, "At the end of my sophomore year, I took a leave of absence. I needed to audition without cutting classes." She also studied at the Lee Strasberg Theatre Institute.

She has appeared in many Off-Broadway productions, including the musical Francis in 1981 at the York Theatre at St. Peter's, The Mystery of Edwin Drood in 1985 at the Public Theater's Delacorte Theatre, Birds of Paradise in 1987 (Promenade Theatre), Privates on Parade (Roundabout Theatre) in 1989, the musical Song of Singapore in 1991, the Michael John LaChiusa musical Hello Again at the Lincoln Center Mitzi Newhouse Theater in 1993, Twelve Dreams at the Mitzi Newhouse Theater in 1995, and Helen at the Public Theater/New York Shakespeare Festival in 2002. In 2012, she appeared in Stephen Sondheim's Into the Woods at The Public Theater's Delacorte Theatre as the Witch.

On Broadway, after They're Playing Our Song (1979), she was an understudy in the musical/opera The Human Comedy in April 1984 and played various roles in The Mystery of Edwin Drood from 1985 to 1987. She also played Audrey Fulquard in Howard Ashman and Alan Menken's Little Shop of Horrors. In 1994, she played the role of Fosca in Stephen Sondheim's and James Lapine's Passion, winning the Tony Award for Best Actress in a Musical for her performance at the 48th Tony Awards. A year later she appeared in Lapine's revival, Twelve Dreams. In 1996, she played Anna Leonowens in the revival of The King and I alongside Lou Diamond Phillips, also recording a cast album. The role earned her a second Tony Award for Best Actress in a Musical at the 50th Tony Awards. She appeared as Ruth Sherwood in a revival of Wonderful Town from 2003 to 2005 (having previously performed in the New York City Center Encores! 2000 staged concert of that musical), and was nominated for the Tony Award for Best Actress in a Musical at the 58th Tony Awards and won the Drama Desk Award for Outstanding Actress in a Musical. In 2007, she appeared in LoveMusik as Lotte Lenya, opposite Michael Cerveris as Kurt Weill, receiving a Tony Award nomination at the 61st Tony Awards as well as a Drama Desk Award nomination. She appeared in the 2007 New York City Center Encores! staged concert of Follies as Phyllis. She appeared in the Roundabout Theatre production of a new musical, The People in the Picture, which opened on April 28, 2011, and closed on June 19, 2011. She was nominated for a 2011 Tony Award for Leading Actress in a Musical for her role in the production at the 65th Tony Awards.

She appeared in the Broadway revival of Hello, Dolly! as Dolly Gallagher Levi, the alternate to Bette Midler on Tuesday evenings and other select performances. She played her last performance on January 7, 2018. Murphy returned to Hello, Dolly! for six performances (July 22 and 29, August 5, 12, 19, and 20) when Midler rejoined the musical before it closed on August 25, 2018.

Murphy's film roles include Anij, Captain Jean-Luc Picard (Patrick Stewart)'s love interest, in Star Trek: Insurrection (1998); in the film Center Stage, as Juliette Simone, a ballet teacher (2000); as Rosalie Octavius, wife of Dr. Otto Octavius (Alfred Molina), the film's villain in Spider-Man 2 (2004); as Betty, a surgical research assistant in Darren Aronofsky's film The Fountain (2006), and Annie Braddock (Scarlett Johansson)'s mother Judy Braddock in The Nanny Diaries (2007). In her voice role as Mother Gothel in the animated musical film Tangled (2010), Murphy sang the song "Mother Knows Best". She also played Kathleen Walker, Corinne Walker (Vera Farmiga)'s mother in Higher Ground (2011) and Marie in Dark Horse (2011). In 2012, she appeared as government secretary Dita Mandy in The Bourne Legacy.

On television, Murphy appeared on the NBC soap opera Another World from 1989 through 1991 as District Attorney Morgan Graves. She won a Daytime Emmy Award for Outstanding Performer in a Children's Special at the 24th Daytime Emmy Awards in 1997, for playing Armanda Agrelo in "Someone Had to be Benny" (1996), an episode of the HBO series Lifestories: Families in Crisis. Other television series roles include a recurring role as Abigail Adams on Liberty! The American Revolution (1997), Murder One (1995–1996), Law & Order as Carla Tyrell in a recurring role (2000), Hack (2002–2003), Trust Me (2009) and Quantico (2017). Her voice-over work in TV commercials, includes the Le Vian chocolate diamonds series for Jared Jewelry. In 2022, Murphy joined the cast of HBO's The Gilded Age as Caroline Schermerhorn Astor.